Together, these findings indicate that ttIL-12 increases CD8+ T cell frequency in tumors and is associated with increases in expression of IFNγ and CXCL9 and that ttIL-12 decreases frequencies of Tregs and MDSCs and is associated with reductions in expression of CCL22 and CXCL2 in the tumor microenvironment. This evidence concerns the gene CXCL9 and neoplasm.