Furthermore, as shown in Fig. 6d, ttIL-12 significantly prolonged survival of OS60-SJ osteosarcoma tumor–bearing mice compared with either Ctrl treatment or wtIL-12 treatment (P = 0.0047 and P = 0.0295, respectively; n = 12~13), which is in line with the results in the other mouse tumor models. The gene discussed is SPRR2A; the disease is osteosarcoma.