As dendritic cells (DCs) are found within the female genital tract (FGT), express DC-SIGN that bind gp120 high mannose N-glycans and facilitate trans-infection of CD4 T cells in the lymph nodes [9, 10], we tested whether matched TF and CI variants from a subtype C single variant transmitted participant differed in mannosylation, binding to DC-SIGN and trans-infection. Here, TF is linked to infection.