In addition to known mutations in genes such as ETV6, NOTCH1, JAK1, and NF1, novel recurrent variants were revealed in FAT1 (25%), FAT3 (20%), DNM2 (35%), and in epigenetic regulators such as MLL2, BMI1, and DNMT3A. A frequency of about 16% of DNMT3A mutations was identified in a larger cohort of adult ETP-ALL patients (10/68). Here, ETV6 is linked to acute lymphoblastic leukemia.