To further study whether VGLL4 participates in miR‐301b‐3p knockdown‐induced inhibition of proliferation, G2/M phase arrest and apoptosis of HCC cells, VGLL4 was knocked down by a specific siRNA in Huh7 cells with miR‐301b‐3p knockdown (P < 0.05, Figure 6A). This evidence concerns the gene VGLL4 and hepatocellular carcinoma.