In our study, the expression levels of SCAMP1, miR‐499a‐5p, LMX1A and NLRC5 were investigated in glioma tissues and cell lines, meanwhile, the effects on regulating malignant progression of glioma and cross‐talk among SCAMP1, miR‐499a‐5p, LMX1A and NLRC5 were completely clarified. This evidence concerns the gene SCAMP1 and central nervous system cancer.