miR‐139 regulated malignant behaviours of glioma cells through directly targeting RUNX1 3′‐UTR.40 miR‐370 was also reported that exerted tumour‐suppressive function in glioma cells by directly binding to CCNE2 3′‐UTR.41 In our study, using bioinformatics database (TargetScan) combined with dual‐luciferase assay certified that LMX1A was directly targeted by miR‐499a‐5p. The gene discussed is CCNE2; the disease is central nervous system cancer.