There was a strong agreement of LPS-specific antibody responses between DBS eluates and plasma of cholera patients (Intraclass correlation coefficient, ICC: IgA = 0.9, IgG = 0.7 and IgM = 0.7; Fig 3A–3C) and vaccinees (ICC: IgA = 0.6, IgG = 0.7 and IgM = 0.8; Fig 4A–4C). This evidence concerns the gene CD40LG and vibrio infectious disease.