Based on our results we found that in well-differentiated HCC AFP levels approximate to a level of about <200 ng/L; in well-moderately differentiated HCC AFP levels are observed at about 200–1600 ng/L; in moderately differentiated HCC AFP levels were about 2000 ng/L; in moderately-poorly differentiated HCC, AFP levels were recorded at 2000–8000ng/l; and in poorly differentiated HCC AFP levels were > 8000ng/L. Here, AFP is linked to hepatocellular carcinoma.