In order to obtain a high-quality set of RNA variants, the following filters with the Vcftools software were applied to the call set of one colon cancer EVs sample, three merged BRCA1-KO fibroblasts samples and three merged exposed BRCA1-KO fibroblast samples: minDP 10, minGQ 10, minQ 30. Here, BRCA1 is linked to colonic neoplasm.