To further explore the correlation between PRAF2 mRNA expression and clinical characteristics in ESCC, patients were divided into the low PRAF2 expression group (n = 41, fold-change ≤ average ratio) and the high PRAF2 expression group (n = 36, fold-change > average ratio) (Fig. 1c). Here, PRAF2 is linked to esophageal squamous cell carcinoma.