Four different human cell lines were arrested in mitosis by treatment with the microtubule polymerization inhibitor nocodazole: the chromosomally unstable, p53-deficient, cervix cancer HeLa cells, the chromosomally stable, p53-proficient, colon cancer HCT116 cells, the lung adenocarcinoma, p53-proficient, A549 cells and the non-cancerous telomerase-immortalized retinal epithelium, p53-proficient, hTERT-RPE1 cells. Here, TP53 is linked to malignant colon neoplasm.