However, ATP6V0d2 is not directly implicated in the macrophage responses related to parasite intracellular multiplication, namely: i) production of nitric oxide (NO) inferred by expression of the inducible isoform of nitric oxide synthase (iNOS, NOS2), the main effector of innate immunity against intracellular pathogens [37]; and ii) expression of arginase, which is involved in polyamine synthesis and is exploited by pathogens to establish intracellular infection [38]. Here, NOS2 is linked to infection.