The activity of enzymes involved in lysosomal storage diseases that could indicate lysosome impairment was also evaluated: lysosomal acid lipase (LAL), implicated in Wolman and cholesteryl ester storage diseases, displayed the same activity in both nonsilenced and ATP6V0d2-KD macrophages; activity of α-galactosidase (α-Gal), implicated in Fabry Disease, was increased in ATP6V0d2-KD macrophages, while β-glucocerebrosidase (GCase) activity, whose activity deficiency is observed in Gaucher Disease, was decreased compared to nonsilenced macrophages (S2C Fig). Here, LIPA is linked to Gaucher disease.