In the present study, twelve candidate markers for BC (SFN (14-3-3σ), HOXA11, ARID1a, CBX7, DLC1, P16, RARβ, PCDHGB7, hMLH1, WNT5a, HOXD13, and RASSF1a) were studied with regard to their detection in BC tissues and matched serum samples. This evidence concerns the gene MLH1 and breast cancer.