The relative expression of JAG2 mRNA in colorectal cancer tissues was determined by quantitative PCR and the results showed that the overall expression of JAG2 in colorectal cancer tissues was increased compared with that of adjacent tissues and the relative content of JAG2 mRNA increased with the clinical stages (N0, N1, and N2) (Fig. 1a), indicating that JAG2 was abnormally expressed in colorectal cancer tissues and directly related to clinical stages of the disease. The gene discussed is JAG2; the disease is colorectal cancer.