Multicellular tumor spheroid culture (MCTS) has been reported to be a more suitable candidate for studying drug penetration due to the high resemblance to solid tumors.18, 19 Here we sought to investigate the role of Gal‐3 in migration, invasion, and chemoresistance of SKOV3 MCTS and to understand its molecular mechanism. This evidence concerns the gene LGALS3 and neoplasm.