In addition, we observed a significant, 5- to 7-fold decrease in the secretion of all analyzed cytokines (CCL2, CCL3, CCL4, TNFα, IL1β, IL6, and IL1RA), except for IL10, by ND2158 treatment of TLR-stimulated CLL cells compared to untreated cells (Fig. 4c). The gene discussed is TNF; the disease is B-cell chronic lymphocytic leukemia.