CLL cells (CD19+CD5+ cell content: 95.1% ± 2.6%) were cultured with TLR agonists and levels of CCL2 (MCP1), CCL3 (MIP1α), CCL4 (MIP1β), TNFα, IL1β, IL6, IL10, IL1RA, IFNγ and IL12-p70 were analyzed in cell culture supernatants using Luminex® Bead Panel. The gene discussed is IL6; the disease is B-cell chronic lymphocytic leukemia.