Results showed AG-490 could not only decrease the number of spheroids and the side population proportion in CD133+ HCC cells (p < 0.01, Fig. 8c, d), but also eliminated the influence of AQP3 overexpression on the stemness of CD133+ HCC cells (p < 0.01, Fig. 8c, d). Here, AQP3 is linked to hepatocellular carcinoma.