AKT1 and neoplasm: Phosphorylation levels of the PI3K effector, AKT/PKB, in TC1 and B16F10 tumor cells were assessed by Western-blot and we indeed observed a strong upregulation of phospho-AKT (pAKT) under GD, OD and especially OGD conditions in the TC1 tumor cell line (Fig. 5a-b).