As shown in Fig. 8 and Additional file 2: Figure S9-S22, the Kaplan-Meier survival analysis revealed that high expression of 14 upregulated genes (Spred3, Nptx1, Angptl2, Adamts14, Meis3, Dlg4, Col6a1, Ltbp2, Cmtm3, Antxr1, Ptges, Fam114a1, Sema4b, or Sned1) significantly reduced the overall survival in patients with renal cancer, urothelial cancer, cervical cancer, pancreatic cancer, colorectal cancer, glioma, liver cancer, ovarian cancer, lung cancer, stomach cancer, thyroid cancer, and endometrial cancer. Here, SPRED3 is linked to central nervous system cancer.