To assess the expression of PD-L1 in NSCLC, we performed analysis of both protein level, by western blot analysis (Fig. 1a-b), and of mRNA level, by RT-qPCR (Fig. 1c), in a panel of NSCLC cell lines, comparing them with BEAS-2B cell line, a human bronchial epithelial model. Here, CD274 is linked to non-small cell lung carcinoma.