CD274 and neoplasm: The effect of anti-PD-L1 agent on cell viability requires immune cells and we demonstrated that the effect was likely due to immune cells killing of tumor cells by repeating the treatment with anti-PD-L1 atezolizumab in EPCAM+ subpopulation of spheroids cultures and confirming they were insensitive, since they lack the autologous immune cells (Fig. 5a).