CCND1 and colorectal carcinoma: qRT-PCR and western blot analysis were then carried out, and results showed that treatment of CDCA2-overexpressing DLD-1 cells with LY294002 for 24 h obviously inhibited p-AKT activity and promoted the decline of CCND1 expression (Fig. 5b and c), indicating that CCND1 accumulation could be suppressed by the addition of inhibitors in CRC cell lines.