To further detect the potential role of CDCA2, we compared the level of CDCA2 mRNA expression between 90 pairs of CRC and adjacent normal tissues using qRT-PCR assay, normalized it to GAPDH (P < 0.001; Fig. 1c), and investigate the connection between the level of CDCA2 expression and the clinical factors of CRC. This evidence concerns the gene GAPDH and colorectal carcinoma.