Four patients in the Group B were diagnosed for AI (true positive): a female patient with hypopituitarism caused by previous pituitary macroadenoma that had been surgically-removed (secondary AI, baseline cortisol 196 nnmol/L, ACTH 5.3 pg/mL), a male patient with thalassemia, hypogonadism and GH deficiency (secondary AI, baseline cortisol 199 nnmol/L, ACTH 28.8 pg/mL), and two female patients with presumed autoimmune adrenalitis (baseline cortisol 28 nmol/L and 135 nmol/L and ACTH were 6.4 pg/mL and 65 pg/mL). This evidence concerns the gene POMC and chronic primary adrenal insufficiency.