NUCB2 and obesity due to melanocortin 4 receptor deficiency: During more experiments on Nesfatin-1 in PVN, Stengel et al. [53] confirmed that the expression quantity of Nucleobindin-2 mRNA and Nesfatin-1 in PVN were evidently decreased in adult rats after 24 h fasting, which proved that Nesfatin-1 in PVN could inhibit the animal feeding process and reduce the incidence of obesity.