To determine whether CSPG4-CAR T cells can react to KOPN8 leukemia cells in an antigen-specific manner, mock T cells, control CEA-CAR T cells, and CSPG4-CAR T cells were co-incubated with T2.A1, KOPN8, and A375M cells at a 1:1 ratio, 24 h after electroporation. The gene discussed is CEACAM5; the disease is leukemia.