A549 is a non-small cell lung carcinoma cell line derived from a primary tumor, and the cells are characterized as pre-alveolar type II pneumocytes of human lung and carry several mutations (i.e., RAS, CDKN2A, FLT3, CBL, KEAP1, ZFHX3, FH, FUS, STK11, ATR, SUFU, HIP1, and SMARCA4). This evidence concerns the gene ZFHX3 and non-small cell lung carcinoma.