The mean tissue level of NFE2L2 was significantly increased in AFB1 induced HCC group compared to the control group, P value was < 0.0001, the mean tissue level of NFE2L2 was significantly raised in C. Sempervirens 300, 600, P. Ginseng 250, 500, N. Sativa 500, 1000 and silymarin groups compared to AFB1 induced HCC group (P value < 0.0001), C. Sempervirens 600 and N. Sativa 1000 increased NFE2L2 level compared to P. Ginseng 500 group (P value was < 0.01) also, Silymarin showed a good response when compared to C. Sempervirens 600 and P. Ginseng 500 groups. This evidence concerns the gene NFE2L2 and hepatocellular carcinoma.