Figure 5B illustrates that FEO significantly up regulated the cleaved caspase 3, caspase 9, and PARP expression in dose-dependent manner. Further time-dependent down regulation of MCL-1 a major anti-apoptotic protein in melanoma was observed in FM94 cells by FEO (10 μg/ml) treatment (Figure 5C). The gene discussed is CASP9; the disease is melanoma.