In view of this, the result of our study showing the involvement of epigenetic mechanisms in the regulation of Tubb2b expression, which was evidenced by simultaneous and progressive Tubb2b over-expression and gene promoter DNA hypomethylation during hepatocarcinogenesis and in HepaRG cells subjected to the oleic acid-induced model of NAFL, are of special interest. The gene discussed is TUBB2B; the disease is non-alcoholic fatty liver.