SYNPO and diabetes mellitus: IL-1β blockade also preserved more podocytes as indicated by either WT-1 staining or WT-1 and synaptopodin mRNA expression (Figures 2G–I), however, it did not alter glomerular tuft area, Bowman's capsule area, or tuft over capsule ratio, three indirect markers of diabetes- and nephron loss-related single nephron hyperfiltration (Supplementary Figure 1A).