Specifically, microfluidic chips compartmentalized with 3D ECM gels containing tumor cells and TCR-T cell loading zones were used to assess the roles of hypoxia, inflammatory cytokines, immunosuppressive conditions induced by mTOR inhibitors, and monocytes on the cytotoxicity of TCR-T cells (20, 21), and microchips filled with ECM gels containing tumor cell spheroids and perfusable tubular vasculatures were used to recapitulate TME for NK cell trafficking toward solid tumors and to test combination of immuno-stimulatory biologics with NK cell therapy (22). This evidence concerns the gene MTOR and neoplasm.