This method of measuring absolute rates of glucose oxidation, which has been applied extensively in various cell types including neurons and astroglia [75, 76], oocytes [77], hepatocytes [78], intestine [79], and heart [80, 81], as well as tumor cells [82, 83], relies upon a set of assumptions that differ significantly from those of the VPDH/VCS method; the similar impact of insulin upon absolute glucose oxidation and VPDH/VCS lends further credence to the latter method. This evidence concerns the gene INS and neoplasm.