These data were validated by use of an entirely independent method, trapping [14CO2] generated by the oxidation of [14C] glucose, which showed that rates of absolute glucose oxidation increase similarly to the change in VPDH/VCS upon insulin stimulation in MC38 colon cancer, 4T1 breast cancer, and TRAMPC3 prostate cancer cells, but are unaltered by insulin in YUMM1.7 melanoma, BCL B cell lymphoma, and NCI-H69 small cell lung cancer cells. The gene discussed is INS; the disease is breast carcinoma.