To explore the functional relevance of AGAP2-AS1 in GBM cells, we interfered endogenous AGAP2-AS1 expression in U87/MG and U251/MG cells by transfection with specific siRNA, and increased AGAP2-AS1 expression in A172 cells by transfection with one overexpression plasmid (Figure 2A). The gene discussed is AGAP2; the disease is glioblastoma.