CCR5 and HIV-1 infection: All together, these results demonstrated that disruption of CCR5 in HIV-1 reporter cell lines by SaCas9/sgRNA-#6 and #8 protected cells from R5-tropic HIV-1 infection and sgRNAs delivered by Lenti-CRISPR/SaCas9 had higher efficiency than AAV- CRISPR/SaCas9.