In this study, we evaluated the effects pirfenidone on TGF-β1-mediated contraction of ECM and migration of lung fibroblasts isolated from patients with lung fibrosis toward fibronectin and compared them with those of normal lung fibroblasts for understanding the mechanisms underlying lung fibroblast-dependent antifibrotic effects of pirfenidone. This evidence concerns the gene TGFB1 and pulmonary fibrosis.