Of the 35 canonical pathways, 16 pathways show > 90% of statistical significance in 1000 bootstrap samples, including many cancer-related signaling pathways such as “Notch-mediated HES/HEY network” (Notch network), “Class I PI3K signaling events mediated by Akt” (PI3K/Akt), “p53 pathway”, “Melanoma”, “Bladder cancer”, “Proteasome”, and “Influence of Ras and Rho proteins on G1 to S Transition” (Ras/Rho on G1 to S). The gene discussed is RHO; the disease is urinary bladder cancer.