Concerning the control groups for the EPISPOT assay in prostate cancer, we already reported data on the detection of PSA-secreting cells (defined as CTCs in prostate cancer) in patients with benign diseases (e.g., acute prostatitis, benign hyperplasia of prostate) as well as in healthy donors [39,40]; we have never observed any positive events in these two control groups. The gene discussed is KLK3; the disease is prostate cancer.