(A) Islet lysates from LepRdb/+ heterozygote (control) or LepRdb/db mice at different stages of diabetes progression (random blood glucose values shown above) were immunblotted for p58ipk and cyclophilin B (CypB, loading control); the graph at right shows the quantitation of the p58ipk / CypB ratio from three independent experiments (mean ± s.d., each point a different animal; asterisk, p=0.05 by Mann Whitney U test and p<0.05 by t-test). This evidence concerns the gene PPIB and diabetes mellitus.