Furthermore, we assessed the effect of IGFBP7 deregulation on anchorage independent growth using soft agar assay, and the results showed that thyroid cancer cells overexpressing IGFBP7 formed fewer and smaller cell colonies in the 3-D setting, and IGFBP7-silenced thyroid cancer cells produced more and larger colonies (Fig. 2f). Here, IGFBP7 is linked to thyroid cancer.