AKT1 and neoplasm: We noted that cell lines resistant to SHOC2 depletion for spheroid growth (A421, H460, A549) have inactivating mutations in the LKB1/STK11 tumour suppressor gene (Supplementary Table 1) as well as retaining higher AKT phosphorylation levels in suspension (Fig. 2i) suggesting possible molecular mechanisms of resistance to SHOC2 ablation for anchorage-independent growth.