Here, calcium imaging was acquired from the neurons in the BC that received afferents from the subcortical thalamic region by using the calcium reporter GCaMP64,5,12; optogenetic stimulation was performed on the VPM thalamic nuclei, after expression of the light-sensitive protein ChR2 (Fig. 4b–d)2,3,32. This evidence concerns the gene TNFSF14 and breast cancer.