To address whether modulation of the Snail/Slug-mediated EMT by API influences the invasive properties of PCa cells, we stably overexpressed Snail and Slug in PC-3 M and DU145 PCa cell lines (Fig. 2d) and demonstrated that overexpression of Snail and Slug caused a significant increase in the invasive ability compared to cells that were transfected with an empty vector. This evidence concerns the gene SNAI1 and posterior cortical atrophy.