In line with recent findings that enhanced invasiveness and clonogenicity characterize CSCs, and that circulating liver CSCs play critical roles in the preparation of new sites for malignant colonization [23,24], and having demonstrated that COL1A1 plays a critical role in the induction and/or enhancement of HCC cells invasion and migration through the deregulation of EMT, in vitro, we investigated probable links between COL1A1-induced metastatic phenotype and CSCs-like phenotype. This evidence concerns the gene COL1A1 and hepatocellular carcinoma.