Expectedly, this siCOL1A1-induced suppressed tumorsphere formation efficacy and loss of self-renewal phenotype was associated with concurrent downregulation of COL1A1, KLF4, OCT4, YAP1 and CD133 in the siCOL1A1 HCC cell lines, compared to their wild-type counterparts (Figure 5C). Here, COL1A1 is linked to hepatocellular carcinoma.