Expectedly, this siCOL1A1-induced suppressed tumorsphere formation efficacy and loss of self-renewal phenotype was associated with concurrent downregulation of COL1A1, KLF4, OCT4, YAP1 and CD133 in the siCOL1A1 HCC cell lines, compared to their wild-type counterparts (Figure 5C). This evidence concerns the gene POU5F1 and hepatocellular carcinoma.