CD133 is one of the most studied CSC biomarkers.6 CD133‐positive glioma cells can grow spheres in serum‐free medium, whereas CD133‐negative cells cannot grow spheres.7, 8 Several studies have demonstrated that knocking down CD133 could weaken tumorigenicity.8 SOX2 is another signature of CSC. The gene discussed is PROM1; the disease is central nervous system cancer.