Chromosomal aberrations are prognostic in neuroblastoma and are correlated with MYCN status.26 To determine the relationship between 5-hmC profiles and copy number, we performed differential 5-hmC analyses between 52 cluster 1 and 37 cluster 2 tumors (Data Supplement) and assessed the chromosomal location of the 3,320 identified genes. This evidence concerns the gene MYCN and neuroblastoma.