Figure4A shows the optical density values of the ELISA results across the range of input CCSP‐2 concentrations, demonstrating clear detection of CCSP‐2 protein but with a lower LOD (0.4 μg mL−1), thus lacking optimal sensitivity for detecting CCSP‐2 protein from the blood of either CRC (red dots) or control (open blue dots) individuals. This evidence concerns the gene VWA2 and colorectal carcinoma.