To shed further light on the mechanism, the protein levels of p-PI3K, p-AKT, p-mTOR, LC3-II, and p62 were tested by Western blot in TNF-α-induced C2C12 myoblasts and skeletal muscles in CKD rats, and reverse validation was performed by the overexpression of Nox2. The gene discussed is TNF; the disease is chronic kidney disease.