To further determine if the cytolytic activity of MUC28z CAR T cells was IFN-γ-dependent, we neutralized IFN-γ with anti-IFN-γ antibody during co-culture, and found that IFN-γ neutralization significantly compromised the tumor lysis ability of MUC28z CAR T cells at E:T ratio of 0.5:1 and 1:1 and trended to compromise lysis at 2:1 of E:T ratio (Figure 4D) suggesting that the lytic potential of the effector cells is partially dependent upon IFN-γ. The gene discussed is IFNG; the disease is neoplasm.