To delineate the role of Dsg3 for pemphigus autoantibody-induced signaling and loss of keratinocyte adhesion, HaCaT cell lines lacking either Dsg3 (ΔDsg3) or Dsg2 (ΔDsg2) were generated using the CRISPR/Cas9 gene editing technology. Here, DSG3 is linked to pemphigus.