As proof of concept, survival data from Buddingh et al. human osteosarcoma (GSE21257) was analyzed for the impact of the original in vivo M1(=LPS+) and in vitro classically activated (LPS+IFN-γ) and in vivo M2(=LPS–) and in vitro alternatively activated (IL-4) gene expression signatures in the tumor biopsy transcriptome from pre-chemotherapy biopsies of osteosarcoma patients. The gene discussed is IL4; the disease is osteosarcoma.