To elucidate the clinical significance of eIF3 subunits expression in gliomas, we systemically analyzed the expression of thirteen eIF3 subunits in 867 gliomas with RNA sequencing and molecular pathological data from the Chinese Glioma Genome Atlas (CGGA) (n = 272) and The Cancer Genome Atlas (TCGA) (n = 595) datasets. This evidence concerns the gene EIF3A and glioma.