However, western blotting showed that miR-708 expression inhibited the expression of SPHK2, phosphorylated Akt, phosphorylated GSK3-β, and β-catenin, which was counteracted by SPHK2 overexpression in LN382 and GBM-GY cells (Fig. 6b). The gene discussed is AKT1; the disease is glioblastoma.